Application

This Anti-CD1d Antibody, clone D5 is validated for use in Western Blotting, Immunoprecipitation for the detection of CD1d.

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Western Blotting Analysis: A representative lot detected time-dependent CD1d degradation in CD1d immunoprecipitate from human penile urethral epithelial (PURL) cells following C. trachomatis infection (Kawana, K., et al. (2007). J Biol Chem. 282(10):7368-7375).
Western Blotting Analysis: A representative lot detected exogenously expressed CD1d in CD1d-transfected C1R human lymphoblastoid cells (Kim, H.S., et al. (1999). J Biol Chem. 274(14):9289-9295).
Immunoprecipitation Analysis: A representative lot immunoprecipitated intact as well as degraded CD1d from human penile urethral epithelial (PURL) cells following C. trachomatis (Kawana, K., et al. (2007). J Biol Chem. 282(10):7368-7375).
Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed CD1d independent of glycosylation states from lysates of CD1d-transfected FO-1 human melanoma cells (Kim, H.S., et al. (1999). J Biol Chem. 274(14):9289-9295).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Antigen-presenting glycoprotein CD1d (UniProt P15813; also known as CD1d, R3G1) is encoded by the CD1D gene (Gene ID 912) in human. CD1d is an MHC-like glycoprotein expressed on epithelial cells and can signal innate immune responses by presenting lipid antigen to natural killer (NK) and NK T (NKT) cells. Similar to MHC class I heavy chain (HC), CD1d HC is glycosylated and assembled with β2m in ER. Unlike MHC class I HC, misfoled CD1d and CD1d not properly assembled with β2m are not destroyed by ER quality control mechanisms. CD1d plays a role in both innate and adaptive immunity to various bacteria, viruses, fungi, and parasites. Activation of CD1d-restricted invariant NKT cells enhances host resistance to these microbes via direct killing of CD1d-expressing (infected) cells or by promoting interferon gamma release from conventional NK cells and by modulating adaptive immune cells via alteration of Th1/Th2 polarization. Chlamydia trachomatis is shown to prevent CD1d-mediated innate immune destruction of the infected urethral epithelial cells by targeting CD1d for proteolytic degradation. Specifically, the chlamydial proteasome-like activity factor (CPAF) interacts with the CD1d HC, and CPAF-associated CD1d HC is then ubiquitinated for eventual degradation.

Immunogen

Pooled CD1d-GST fusion proteins containing α1 domain alone, α1-α2 domains, or α1–α3 domains.

Epitope: CD1d α1 domain

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in THP-1 cell lysate.

Western Blotting Analysis: 4.0 µg/mL of this antibody detected CD1d in 10 µg of THP-1 cell lysate.
Note: Longer exposure time is needed when using total lysates for Western blotting applications. Membrane preparation or immunoprecipitation can be employed to enrich membrane target proteins prior to Western blotting.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~37/43 kDa observed